In this study, we measured the activity of the erythrocyte pyrimidine 5/-nucleotidase(P5N) for 83 workers exposed to lead in their workplace, and analyzed the correlation of the activity with other biological exposure indices of lead. The
measurement
was performed by using a high performance liquid chromatography (HPLC) with a reverse phased ODS column.
@ES The results are as follows:
@EN 1) The correlation of the erythrocyte P5N activity with the concentration of blood lead(PbB) was shown to be statistically significant (r=-0.71, p=0.0001).
2) For a group of subjects whose PbB was less than 10 μg/dl, the erythrocyte P5N activity was 14.9±1.5μmole uridine/h/g Hb.
3) For medical surveillance, this study suggest the erythrocyte P5N activity of 12 μmole uridine/h/g Hb is equivalent to 40μg/dl of PbB
4) The correlation coefficients of the erythrocyte P5N activity with other biological exposure indices of lead such as PbB, ZPP, ALA-U, PBU, CP-U, ALAD, and log ALAD were -0.71, -0.64, 0.57, -0.51, -0.50, 0.46, and 0.64, respectively.
5) The correlation coefficients of the PbB with other biological exposure indices of lead for ALAD, P5N, ZPP, PBU, CP-U, and ALA-U were -0.76, -0.71, 0.68, 0.59, 0.42, and 0.41, respectively.
The erythrocyte P5N activity can be used as a reliable biological exposure index of lead.
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